Wat Am Mae was situated on the city island of Ayutthaya in the south in Pratu Chai sub-district. The monastery was located on the west bank of Khlong Pratu Thep Mi, which connected the old Lopburi River (at present Khlong Muang or City Canal) in the north with the Chao Phraya River in the south at Thep Mi water gate.
In the vicinity of the temple was the Saphan Thep Mi, an old bridge, of which some of it remains are still visible. Wat Borom Phuttharam lie on its east, while Wat Pa Jan stood on its south. To its north was Wat Pa Rong, located along the same canal.
There are no traces anymore visible of the monastery above ground level.
Historical data about the monastery and its construction are unknown.