Historical data about the monastery and its construction are unknown.
In the 'Description of Ayutthaya', it is stated that there was a land market in Iron Village at Tha Khlong. In the village, braziers for forging iron nails and cramps, large and small, were sold. [4]
Wat Tha Khlong was situated at the geographical coordinates: 14° 22' 8.53" N, 100° 34' 11.20" E.
Another temple with the same name exists north of Ayutthaya, in the Wat Tum area.
Footnotes:
(1) In the mid-16th century, the Phaniat peninsula was still attached to Ayutthaya, and the Lopburi River did not yet surround Ayutthaya. Around 1580 CE, a shunt was dug from the Lopburi River near the Khu Na, or front moat, so that the Lopburi River completely enveloped Ayutthaya and separated the Phaniat area from the city. Shortly after, large wooden trunks were used as a weir (Thamnop Ro) and plugged into the shunt to prevent the northern side from becoming shallow, which would have left the Grand Palace area vulnerable to attack. From then on, the Lopburi River encircled the city of Ayutthaya. Hua Ro became the northern point of Ayutthaya.
References:
[1] Baker, Chris (2011). Note On Testimonies And Description Of Ayutthaya. Journal of the Siam Society. Vol. 99.
[2] Baker, Chris (2014). Final Part of the Description of Ayutthaya with Remarks on Defense, Policing, Infrastructure, and Sacred Sites. Journal of the Siam Society, Vol. 102.
[3] Cushman, Richard D. & Wyatt, David K. (2006). The Royal Chronicles of Ayutthaya. Bangkok: The Siam Society. p. 24.
[4] Baker, Chris (2011). Before Ayutthaya Fell: Economic Life in an Industrious Society. Markets and Production in the City of Ayutthaya before 1767: Translation and Analysis of Part of the Description of Ayutthaya. Journal of the Siam Society. Vol. 99.